Composite

Part:BBa_K5226087:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-30)


PR/PRM -tnaA -fmo


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1960
    Illegal PstI site found at 724
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1960
    Illegal PstI site found at 724
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1960
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1960
    Illegal PstI site found at 724
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1960
    Illegal PstI site found at 724
    Illegal NgoMIV site found at 1912
    Illegal AgeI site found at 1347
    Illegal AgeI site found at 2056
    Illegal AgeI site found at 2386
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

tnaA is obtained through PCR amplification.Sequence source is from the genome of E.coli MG1655. fmo is obtained through synthetic（BGI）. Sequence source is from Methylophaga aminisulfidivorans, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/AF494423）

References